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Método Taguchi para optimizar la amplificación de la región código de barras de ADN en moscas de importancia forense (Diptera: Calliphoridae)
Taguchi method in the optimization of the DNA barcode region amplification in forensic important flies (Diptera: Calliphoridae)
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Gómez, G. F., Londoño, A. F., & Maya, A. F. (2018). Método Taguchi para optimizar la amplificación de la región código de barras de ADN en moscas de importancia forense (Diptera: Calliphoridae). Memorias Forenses, (1), 45–52. https://doi.org/10.53995/25390147.460
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Jan 1, 2018
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2018-01-01 (1)
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La estandarización de técnicas moleculares como la reacción en cadena de la polimerasa para la amplificación de la región código de barras constituye un paso crucial en el laboratorio hacia la implementación de esta estrategia en moscas de importancia forense. Tradicionalmente, la búsqueda de condiciones óptimas requiere la ejecución de un gran número de experimentos independientes que varían en las concentraciones de los componentes de la reacción. En este estudio se implementó la metodología Taguchi para establecer las condiciones óptimas usando un número mínimo de experimentos en moscas de la familia Calliphoridae. La cantidad de ADN, así como la temperatura de alineamiento de los cebadores representaron los factores más relevantes en el éxito de la amplificación. Esta metodología puede aplicarse para la estandarización de otras técnicas moleculares como una estrategia simple, rápida y de bajo costo.
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Referencias (VER)
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Ratnasingham, S., & Hebert, P. D. N. (2007). BOLD: The Barcode of Life Data System: Barcoding. Molecular Ecology Notes, 7, 355-364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
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Taguchi, G., & Konishi, S. (1987). Taguchi Methods: Orthogonal Arrays and Linear Graphs. Dearborn, Michigan, USA: American Supplier Institute.
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Watts, P. C., Thompson, D. J., Allen, K. A., & Kemp, S. J. (2007). How useful is DNA extracted from the legs of archived insects for microsatellite-based population genetic analyses? Journal of Insect Conservation, 11(2),
195-198. https://doi.org/10.1007/s10841-006-9024-y
Wells, J. D., & Sperling, F. a H. (2001). DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae). Forensic Science International, 120(1-2),
110-115. https://doi.org/10.1016/S0379-0738(01)00414-5
Wells, J. D., & Stevens, J. R. (2008). Application of DNA-based methods in forensic entomology. Annual Review of Entomology, 53, 103-120. https://doi.org/10.1146/annurev.ento.52.110405.091423
Whitworth, T. (2014). A revision of the Neotropical species of Lucilia Robineau-Desvoidy (Diptera: Calliphoridae). Zootaxa (Vol. 3810). https://doi.org/10.11646/zootaxa.3810.1.1
Wilfinger, W. W., Mackey, K., & Chomczynski, P. (1997). Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. BioTechniques, 22(3),
474-481.
Amat, E., Vélez, M. C., & Wolff, M. (2008). Clave ilustrada para la identificación de los géneros y las especies de califóridos (Diptera: Calliphoridae) de Colombia. Caldasia, 30(1),
231-244.
Amendt, J., Richards, C. S., Campobasso, C. P., Zehner, R., & Hall, M. J. R. (2011). Forensic entomology: Applications and limitations. Forensic Science, Medicine, and Pathology, 7(4),
379-392. https://doi.org/10.1007/s12024-010-9209-2
Asghar, U., Malik, M. F., Anwar, F., Javed, A., & Raza, A. (2015). DNA Extraction from Insects by Using Different Techniques: A Review. Advances in Entomology, 3, 132-138.
Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. a, €¦ Blackshaw, S. (2003). Current Protocols in Molecular Biology. Molecular Biology (Vol. 1). New York. https://doi.org/10.1002/mrd.1080010210
Ball, S. L., & Armstrong, K. F. (2008). Rapid, one-step DNA extraction for insect pest identification by using DNA barcodes. Journal of Economic Entomology, 101(2),
523-532.
Bartlett, J. M. S., & Stirling, D. (2003). A short history of the polymerase chain reaction. Methods in Molecular Biology, 226, 3-6. https://doi.org/10.1385/1-59259-384-4:3
Benecke, M. (2001). A brief history of forensic entomology. Forensic Science International, 120(1-2),
2-14. https://doi.org/10.1016/S0379-0738(01)00408-X
Celani de Souza, H. J., Borges Silva, M., Moyses, C. B., Lopes Alberto, F., Pontes, F. J., Ferreira, U. R., €¦ Sanches da Silva, C. E. (2013). Robust Design and Taguchi Method Application. En M. Borges Silva (Ed.),
Design of Experiments - Applications (pp. 1-20). InTech.
Cobb, B. D., & Clarkson, J. M. (1994). A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods. Nucleic Acids Research, 22(18),
3801-3805. https://doi.org/10.1093/nar/22.18.3801
Condamine, F. L., Clapham, M. E., & Kergoat, G. J. (2016). Global patterns of insect diversification: towards a reconciliation of fossil and molecular evidence? Scientific Reports, 6, 19208. https://doi.org/10.1038/srep19208
Dean, M. D., & Ballard, J. W. O. (2001). Factors affecting mitochondrial DNA quality from museum preserved Drosophila simulans. Entomologia Experimentalis et Applicata, 98(3),
279-283.
Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294-299. https://doi.org/10.1371/journal.pone.0013102
Gutiérrez-López, R., Martínez-de la Puente, J., Gangoso, L., Soriguer, R. C., & Figuerola, J. (2015). Comparison of manual and semi-automatic DNA extraction protocols for the barcoding characterization of hematophagous louse flies (Diptera: Hippoboscidae). Journal of Vector Ecology, 40(1),
11-15. https://doi.org/10.1111/jvec.12127
Hebert, P. D. N., Cywinska, A., Ball, S. L., & DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings. Biological Sciences / The Royal Society, 270, 313-321. https://doi.org/10.1098/rspb.2002.2218
Joseph, I., Mathew, D., Sathyan, P., & Vargheese, G. (2011). The use of insects in forensic investigations: An overview on the scope of forensic entomology. Journal of Forensic Dental Sciences, 3(2),
89-91. https://doi.org/10.4103/0975-1475.92154
Nakano, A., & Honda, J. (2015). Use of DNA sequences to identify forensically important fly species and their distribution in the coastal region of Central California. Forensic Science International, 253, 1-13. https://doi.org/10.1016/j.forsciint.2015.05.001
Neumaier, M., Braun, A., & Wagener, C. (1998). Fundamentals of quality assessment of molecular amplification methods in clinical diagnostics. Clinical Chemistry, 44(1),
Packer, L., Gibbs, J., Sheffield, C., & Hanner, R. (2009). DNA barcoding and the mediocrity of morphology. Molecular Ecology Resources, 9(Suppl. 1),
42-50. https://doi.org/10.1111/j.1755-0998.2009.02631.x
Promega. (2016). PCR Amplification. Recuperado de https://worldwide.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/pcr-amplification/
Rao, R. S., Kumar, C. G., Prakasham, R. S., & Hobbs, P. J. (2008). The Taguchi methodology as a statistical tool for biotechnological applications: A critical appraisal. Biotechnology Journal. https://doi.org/10.1002/biot.200700201
Ratnasingham, S., & Hebert, P. D. N. (2007). BOLD: The Barcode of Life Data System: Barcoding. Molecular Ecology Notes, 7, 355-364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
Sambrook, J., Fritsch, E. F., & Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. New York (3rd ed.). New York: Cold Spring Harbor Laboratory Press. https://doi.org/574.873224 1/1989
Schwarz, C., Debruyne, R., Kuch, M., Mcnally, E., Schwarcz, H., Aubrey, A. D., €¦ Poinar, H. (2009). New insights from old bones: DNA preservation and degradation in permafrost preserved mammoth remains. Nucleic Acids Research, 37(10),
3215-3229. https://doi.org/10.1093/nar/gkp159
Sharma, P., & Kobayashi, T. (2014). Are “universal” DNA primers really universal? Journal of Applied Genetics, 55(4),
485-496. https://doi.org/10.1007/s13353-014-0218-9
Sperling, F. A., Anderson, G. S., & Hickey, D. A. (1994). A DNA-based approach to the identification of insect species used for postmortem interval estimation. Journal of Forensic Sciences, 39(2),
418-427.
Swinehart, D. F. (1962). The Beer-Lambert Law. Journal of Chemical Education, 39(7),
333. https://doi.org/10.1021/ed039p333
Taguchi, G., & Konishi, S. (1987). Taguchi Methods: Orthogonal Arrays and Linear Graphs. Dearborn, Michigan, USA: American Supplier Institute.
Watson, J. D., & Crick, F. H. C. (1953). Molecular structure of nucleic acids. Nature. https://doi.org/10.1097/BLO.0b013e3181468780
Watts, P. C., Thompson, D. J., Allen, K. A., & Kemp, S. J. (2007). How useful is DNA extracted from the legs of archived insects for microsatellite-based population genetic analyses? Journal of Insect Conservation, 11(2),
195-198. https://doi.org/10.1007/s10841-006-9024-y
Wells, J. D., & Sperling, F. a H. (2001). DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae). Forensic Science International, 120(1-2),
110-115. https://doi.org/10.1016/S0379-0738(01)00414-5
Wells, J. D., & Stevens, J. R. (2008). Application of DNA-based methods in forensic entomology. Annual Review of Entomology, 53, 103-120. https://doi.org/10.1146/annurev.ento.52.110405.091423
Whitworth, T. (2014). A revision of the Neotropical species of Lucilia Robineau-Desvoidy (Diptera: Calliphoridae). Zootaxa (Vol. 3810). https://doi.org/10.11646/zootaxa.3810.1.1
Wilfinger, W. W., Mackey, K., & Chomczynski, P. (1997). Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. BioTechniques, 22(3),
474-481.
